Intact plasma membranes in dried cells
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of plasma membranes and intact nuclei from lymphoid cells.
A method has been developed for the rapid large scale isolation of plasma membranes and intact nuclei from RAJI lymphoid cells utilizing hypotonic lysis of cells after intracellular loading with glycerol followed by combined flotation-sedimentation within a discontinuous sucrose gradient. Nuclei may be isolated in about 1 h and plasma membranes in about 6 h from 1 to 20 g of cells. Intact nucle...
متن کاملPlasma Membranes of Mammalian Cells
The plasma membrane surrounding living cells "plays a role in intracellular metabolism, but what distinguishes this structure from other organelles is that the plasma membrane also mediaces interactions between the cell and its external environment. The simplest of these interactions maintain a desirable intracellular milieu by bringing in needed substances and getting rid of waste products. Th...
متن کاملPlasma membranes from insect midgut cells.
Plasma membranes from insect midgut cells are separated into apical and basolateral domains. The apical domain is usually modified into microvilli with a molecular structure similar to other animals. Nevertheless, the microvillar structure should differ in some insects to permit the traffic inside them of secretory vesicles that may budd laterally or pinch-off from the tips of microvilli. Other...
متن کاملModulation of 5'-nucleotidase activity in plasma membranes and intact cells by the extracellular matrix proteins laminin and fibronectin.
Modulation of 5'-nucleotidase activity by the extracellular matrix proteins fibronectin, laminin and their fragments has been studied in plasma membrane preparations as well as in intact BCS-TC2 and Rugli cells. The ectoenzyme on plasma membranes is activated by laminin; fibronectin inhibits the AMPase activity on BCS-TC2 plasma membranes but no inhibitory effect is found in plasma membrane pre...
متن کاملFunctional testosterone receptors in plasma membranes of T cells.
T cells are considered to be unresponsive to testosterone due to the absence of androgen receptors (AR). Here, we demonstrate the testosterone responsiveness of murine splenic T cells in vitro as well as the presence of unconventional cell surface receptors for testosterone and classical intracellular AR. Binding sites for testosterone on the surface of both CD4(+) and CD8(+) subsets of T cells...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: South African Journal of Botany
سال: 2007
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2007.04.023